MILK AMINO ACIDS

MILK AMINO ACIDS - Names and Identifiers

Name	amicase
Synonyms	MILK AMINO ACIDS
CAS	65072-00-6
EINECS	265-363-1

MILK AMINO ACIDS - Physico-chemical Properties

Molecular Formula	C21H41N5O11
Molar Mass	539.57714
Melting Point	280 °C
Water Solubility	Soluble in water.
Solubility	310g/l
Appearance	Off-white to beige powder
Color	Off-White to beige
Odor	Odorless
PH	4.7-5.7 (50g/l, H2O, 20℃)
Storage Condition	+15C to +30C
Sensitive	Highly hygroscopic
Use	Bacteriological medium to provide growth factors required for bacterial growth

MILK AMINO ACIDS - Risk and Safety

WGK Germany	3
RTECS	FI3519450
TSCA	Yes
HS Code	3504 00 90

MILK AMINO ACIDS - Reference

Reference

1. [IF=9.147] Lixin Wang et al."Ability of casein hydrolysate-carboxymethyl chitosan conjugates to stabilize a nanoemulsion: Improved freeze-thaw and pH stability."Food Hydrocolloid. 2020 Apr;101:105452
2. [IF=7.514] Xiaofei Li et al."Acid-induced mixed methylcellulose and casein gels: Structures, physical properties and formation mechanism."Food Chem. 2022 Jan;366:130561
3. [IF=2.881] Chen Li et al."Development of Chitosan/Peptide Films: Physical, Antibacterial and Antioxidant Properties."Coatings. 2020 Dec;10(12):1193

MILK AMINO ACIDS - Introduction

Moisture: 5 ~ 6%; Total nitrogen content: 7 ~ 8%; Amino nitrogen content: 4 ~ 8%; Chloride: 28~40%;pH:5~7 acid hydrolysate of casein is decomposed to the degree of amino acid, in which there are basically no other nutrients and growth factors except nutritional proteins. This product can be used as a culture substrate used in basic disciplines such as microbiology and genetics. Using casein as raw material, the milky white to light yellow powder is refined by acid hydrolysis activated carbon decolorization treatment, which is easy to absorb moisture. Soluble in water, insoluble in ether and methanol

Last Update：2022-10-16 17:27:44

MILK AMINO ACIDS - Reference Information

EPA chemical substance information	information provided by: ofmpeb.epa.gov (external link)
Introduction	acid hydrolyzed casein is high quality casein as raw material, with strong acid depth hydrolysis, decolorization, desalination, concentration, spray-dried white or yellowish powder. It is easy to absorb moisture, soluble in water, with sauce flavor, is an acidic decomposition of casein, can be decomposed to the extent of amino acids. Acid hydrolyzed casein is casein and its related products through strong acid hydrolysis, decolorization, neutralization, desalination, drying and other processes to prepare a product, which is mainly composed of amino acids and short peptides. Acid hydrolyzed casein according to product purity (I. E., chloride content) is mainly divided into industrial grade (chloride content higher than 3%) and pharmaceutical grade (chloride content lower than 3%) two categories.
Use	1. In the pharmaceutical industry, acid hydrolyzed casein is mainly used as a raw material of pharmaceutical products. In the food industry, acid hydrolyzed casein is mainly used as a high-end nutritional additives. 3. In the daily chemical industry, acid hydrolyzed casein can be used as amino acid skin care products, amino acid products of raw materials; 4. In the fermentation industry, acid hydrolyzed casein is mainly used for microbial culture medium; 5. In the feed industry, acid hydrolyzed casein can be used as a feed additive to strengthen the nutrition of animal feed. In the plant fertilizer industry, acid hydrolyzed casein is an important raw material for amino acid Metal chelate fertilizer. Thus, acid hydrolysis of casein has a very wide range of applications.
application field	acid hydrolyzed casein is widely used in pharmaceutical, biological fermentation, animal feed, plant tissue culture, cosmetics, tobacco industry (flavor), food hygiene inspection and other professional and various types of medium configuration, especially the configuration of various types of semi-integrated medium, this product is the main basic components, such: semi-comprehensive pertussis medium, semi-comprehensive epidemic cerebrospinal meningitis medium, semi-comprehensive liquid culture medium for polyglucosidase, Staphylococcus aureus protein a medium, thioglycollate sterility test medium, anaerobic bacteria medium and activated carbon agar medium, etc, are based on acid hydrolysis of casein.
preparation	1. Hydrolysis: accurately weigh 100g of casein into 1000ml three-necked flask, then add 100ml of distilled water and stir evenly, 100ml of 12mol/L concentrated hydrochloric acid and stir evenly, install condenser tube, electric stirring, heating sleeve, while stirring, when the temperature reaches 90 ℃, the whole solution boiling, continuous constant temperature stirring hydrolysis 3hr, after the solution hydrolysis completely without casein particles to stop the hydrolysis. 2. Filtration: 100ml of distilled water was added to the hydrolysis solution obtained in step 1, and the mixture was filtered with 80 mesh nylon mesh. 3. Concentration: The above-mentioned filtrate was added to a 1000ml round-bottom flask in portions, and concentrated to a paste form under reduced pressure to 650mmHg by a rotary evaporator. 100ml of distilled water was added under negative pressure to reduce the rotary evaporator rotation speed and completely dissolved. Then, it was concentrated under reduced pressure again to a paste form, and the washing was completed by repeating the concentration and washing three times in this way. At this time, the pH value was 3.5. 4. Decolorization: the above-mentioned washed hydrolysate is added to activated carbon with a mass fraction of solid content of 5% and refluxed for 4.0 hours, and filtered with a suction filter bottle and a Buchner funnel under thermal pressure of 2.0 mmHg. The filtrate is pale yellow and the pH value is. 5. Deacidification: the light yellow filtrate After decolorization is exchanged with a strong basic anion 201 × 7(717) resin in one step, and the components of the exchange solution at pH 6.5-7.0 are collected. 6. Drying: After the solution is concentrated by resin exchange and spray dried at a pressure of 15MPa and a temperature of 120 ° C., the product obtained is milky white or light yellow.
medicinal use	in the pharmaceutical industry, acid hydrolyzed casein is mainly used as a raw material of pharmaceutical products, such as casein oral liquid, amino acid Metal chelate preparation; In the food industry, acid hydrolyzed casein is mainly used as a high-end nutritional additives, used to supplement amino acids; In the daily chemical industry, acid hydrolyzed casein can be used as raw materials for amino acid skin care products and amino acid daily chemical products; In the fermentation industry, acid hydrolyzed casein is mainly used for microbial culture medium; In the feed industry, acid hydrolyzed casein can be used as a feed additive to strengthen the nutrition of animal feed. In the plant fertilizer industry, acid hydrolyzed casein is an important raw material of amino acid Metal chelate fertilizer. it can be seen that acid hydrolysis of casein has a very wide range of uses.
Current situation	acid hydrolyzed casein is mainly divided into industrial grade (chloride content is higher than 3%) according to product purity (chloride content) and pharmaceutical grade (chloride content less than 3%) two categories. However, due to the limitation of desalting process, the salt content in acid hydrolyzed casein products is too high and the purity of the products is low. at present, the acid hydrolyzed casein products on the market in our country are all industrial grade, in which the chloride content is as high as more than 20%, which can not meet the requirements of the food industry and pharmaceutical industry. although the chloride content can be reduced by the purification of a specific type of resin, the existing process is complicated, and the recovery rate of the acid hydrolyzed casein product is too low, which is difficult to realize industrialization, or the purity of the product is still difficult to meet the requirements of pharmaceutical grade. For the above reasons, the application of acid hydrolysis of casein is greatly limited.